Interleukin-5, Rat Recombinant, produced in
Escherichia coli is a dimeric, non-glycosylated polypeptide chain containing 113 amino acids and having a molecular mass of 13 kDa. The IL-5 is purified by proprietary chromatographic techniques.
Synonyms: EDF, BCDFII, TRF, T-cell replacing factor, Eosinophil differentiation factor, B cell differentiation factor I, IL-5.
Formulation: Lyophilized from a concentrated (1mg/ml) solution in water containing no additives.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Solubility: It is recommended to reconstitute the lyophilized Interleikin-5 Rat in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability: Lyophilized Interleukin-5 Rat although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL5 Rat should be stored at +4°C between 2-7 days and for future use below -18°C. Please avoid freeze thaw cycles.
Biological Activity: The ED50 range=0. 3-1.0 ng/ml as determined by the dose-dependant stimulation of the proliferation of BCL-1 cells.
Source:Escherichia coli. Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Ile-Pro-Met.
Uniprot ACC#: Q08125
Transportation method: Shipped at Room temperature
Safety data sheet: Inquire at info@3hbiomedical.com