Interleukin-15, Rat Recombinant, produced in
Escherichia coli is a single, non-glycosylated polypeptide chain containing 115 amino acids and having a molecular mass of 13.5 kDa. The IL-15 is purified by proprietary chromatographic techniques.
Synonyms: IL-15, MGC9721.
Formulation: The protein was lyophilized from 10mM Tris, pH-8. 5.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 98% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Solubility: It is recommended to reconstitute the lyophilized Interleukin-15 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability: Lyophilized Interleukin-15 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL15 should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The ED50 as determined by the dose-dependant stimulation of the proliferation of CTLL-2 was found to be < 10 ng/ml, corresponding to a Specific Activity of 100,000 IU/mg.
Source:Escherichia coli. Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Asn-Trp-Ile-Asp.
Uniprot ACC#: P97604
Transportation method: Shipped at Room temperature
Safety data sheet: Inquire at info@3hbiomedical.com