IGF-1 Rat Recombinant produced in
Escherichia coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7.7 kDa. IGF-I is purified by proprietary chromatographic techniques.
Synonyms: Somatomedin C, IGF-I, IGFIA, IGF1.
Formulation: The protein was lyophilized with a 0.2 µm filtered concentrated solution in 20mM PBS, pH 7. 0.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 98% as determined by SDS-PAGE.
Solubility: It is recommended to reconstitute the lyophilized IGF1 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability: Lyophilized IGF-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IGFI should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The ED50 as determined by a cell proliferation assay using FDC-P1 cells is less than 2. 0ng/ml, corresponding to a specific activity of >500,000units/mg.
Source:Escherichia coli. Amino acid sequence: GPETLCGAEL VDALQFVCGP RGFYFNKPTG YGSSIRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPTKSA.
Uniprot ACC#: P08025
Transportation method: Shipped at Room temperature
References: Title:Evidence that androgen-independent stromal growth factor signals promote androgen-insensitive prostate cancer cell growth in vivo. Publication: Published online before print March 17, 2009, doi: 10.1677/ERC-08-0219 Endocr Relat Cancer June 1, 2009 16 415-428 . Link:http://erc. endocrinology-journals. org/content/16/2/415. full
Safety data sheet: Inquire at info@3hbiomedical.com