Epidermal Growth Factor, Rat Recombinant, produced in
Escherichia coli is a single, non-glycosylated, polypeptide chain containing 53 amino acids and having a molecular mass of 6.1 kDa. The Rat EGF is purified by proprietary chromatographic techniques.
Synonyms: Urogastrone, URG, EGF.
Formulation: Rat EGF was lyophilized from a 0.2 µm filtered concentrated (1.0mg/ml) solution in PBS, pH 7.4.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 98% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Solubility: It is recommended to reconstitute the lyophilized Rat EGF in sterile water not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability: Lyophilized Rat EGF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Rat EGF should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The ED50 as calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells is less than 0.1ng/ml, corresponding to a specific activity of > 10,000,000 units/mg.
Source:Escherichia coli. Amino acid sequence: NSNTGCPPSY DGYCLNGGVC MYVESVDRYV CNCVIGYIGE RCQHRDLRWW KLR.
Uniprot ACC#: P07522
Transportation method: Shipped at Room temperature
Safety data sheet: Inquire at info@3hbiomedical.com