Eotaxin, Rat Recombinant, produced in
Escherichia coli is a single, non-glycosylated polypeptide chain containing 74 amino acids and having a molecular mass of 8.4 kDa. The CCL11 is purified by proprietary chromatographic techniques.
Synonyms: Small inducible cytokine A11, CCL11, Eosinophil chemotactic protein, chemokine (C-C motif) ligand 11, SCYA11.
Formulation: Filtered (0.2 µm) and lyophilized from a concentrated (1mg/ml) solution in in 1×PBS, pH 7.4.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 97% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Solubility: It is recommended to reconstitute the lyophilized Eotaxin in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability: Lyophilized Eotaxin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CCL11 should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: Determined by its ability to chemoattract purified blood eosinophils using a concentration range of 0.1-1.0 µg/ml, corresponding to a Specific Activity of 10-100 IU/mg.
Source:Escherichia coli. Amino acid sequence: HPGSIPTSCC FTMTSKKIPN TLLKSYKRIT NNRCTLKAIV FKTKLGKEICADPKKKWVQD ATKHLDQKLQ TPKP.
Uniprot ACC#: P97545
Transportation method: Shipped at Room temperature
Safety data sheet: Inquire at info@3hbiomedical.com