Cardiotrophin-1, Rat Recombinant, produced in
Escherichia coli is a single, non-glycosylated, polypeptide chain containing 203 amino acids and having a molecular mass of 21.4 kDa. The CTF1 Rat is purified by proprietary chromatographic techniques.
Synonyms: Cardiotrophin-1, CT-1, Ctf1.
Formulation: Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as determined by SDS-PAGE and HPLC analyses.
Solubility: It is recommended to reconstitute the lyophilized CTF1 in sterile 4mM HCl not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability: Lyophilized Cardiotrophin-1 Rat although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CTF1 Rat should be stored at +4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Biological Activity: The ED50 as determined by the dose-dependent proliferation of TF-1 cells was found to be < 0.5 ng/ml, corresponding to a specific activity of > 2. 0×106 units/mg.
Source:Escherichia coli. Amino acid sequence: MSQREGSLED HQTDSSFSFL PHLEAKIRQT HNLARLLTKY ADQLLEEYVQ QQGEPFGLPG FSPPRLPLAG LSGPAPSHAG LPVSERLRQD AAALSALPAL LDAVRRRQAE LNPRAPRLLR SLEDAARQVR ALGAAVETVL AALGAAARGP VPEPVATSAL FTSNSAAGVF SAKVLGLHVC GLYGEWVSRT EGDLGQLVPG GVA.
Uniprot ACC#: Q63086
Transportation method: Shipped at Room temperature
Safety data sheet: Inquire at info@3hbiomedical.com