C-10, Rat Recombinant, produced in
Escherichia coli is a single, non-glycosylated, polypeptide chain containing 94 amino acids and having a total molecular mass of 10.4 kDa. C-10 is purified by proprietary chromatographic techniques.
Synonyms: Small inducible cytokine A6, CCL6, C10 protein, c10, MRP-1, Scya6, chemokine (C-C motif) ligand 6.
Formulation: Lyophilized from a 0.2 µm filtered concentrated (1.0mg/ml) solution in 1×PBS, pH 7.4.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Solubility: It is recommended to reconstitute the lyophilized C-10 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability: Lyophilized C-10 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution C-10 should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: Determined by its ability to chemoattract human CCR1 transfected BaF3 mouse proB cells using a concentration range of 0.05-0.25 µg/ml.
Source:Escherichia coli. Amino acid sequence: GLIQDTVKED RPFNPTIIHQ GFQDSSDCCF SYASQIPCSR FIYYFPTSGG CTKPGIIFVT RKRKRVCANP SDQRVQTCIS TLKLGPRSGN SAIA.
Uniprot ACC#: Q68FP3
Transportation method: Shipped at Room temperature
Safety data sheet: Inquire at info@3hbiomedical.com