The BRAK (CXCL14) is involved in immunoregulatory and inflammatory processes. CXCL14 displays chemotactic activity for monocytes but not for lymphocytes, dendritic cells, neutrophils or macrophages.
Recombinant Rat BRAK (CXCL14) is produced in Escherichia coli and it is a single, non-glycosylated, Polypeptide chain containing 77 amino acids and having a molecular mass of 9.4 kDa.
The CXCL14 is purified by proprietary chromatographic techniques.
Synonyms
C-X-C motif chemokine 14, B-cell and monocyte-activating chemokine, Chemokine BRAK, Kidney-expressed chemokine CXC, MIP-2G, Small-inducible cytokine B14, Cxcl14, Bmac, Kec, Ks1, Mip2g, Scyb14, BRAK, NJAC, AI414372, bolekine, MIP2gamma, 1110031L23Rik, 1200006I23Rik.
Formulation
CXCL14 was lyophilized from a 0.2 µm filtered concentrated solution in 30% Acetonitrile and 0.1% TFA.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Solubility
It is recommended to reconstitute the lyophilized CXCL14 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability
Lyophilized CXCL14 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C.
Upon reconstitution CXCL14 should be stored at +4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Biological Activity
Determined by its ability to chemoattract activated monocytes using a concentration range of 1.0-10 ng/ml.
Source
Escherichia coli.
Amino acid sequence
SKCKCSRKGP KIRYSDVKKL EMKPKYPHCE EKMVIITTKS MSRYRGQEHC LHPKLQSTKR FIKWYNAWNE KRRVYEE.
Uniprot ACC#
Q8K453
Shipping condition
Shipped at Room temperature
Safety data sheet
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