BD-3, Rat Recombinant, produced in
Escherichia coli is a single, non-glycosylated polypeptide chain containing 41 amino acids and having a molecular mass of 4.5 kDa. The BD-3 is purified by proprietary chromatographic techniques.
Synonyms: Beta-defensin 3, BD-3, Defensin beta 3, Defb3.
Formulation: BD-3 protein was lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 98% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Solubility: It is recommended to reconstitute the lyophilized BD-3 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability: Lyophilized BD-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BD-3 should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: Measured by its antimicrobial activity against
E. coli. The ED50 for this effect is typically 4-20µg/ml, corresponding to a specific activity of 50,000-250,000units/mg.
Source:Escherichia coli. Amino acid sequence: KKVYNAVSCM TNGGICWLKC SGTFREIGSC GTRQLKCCKK K.
Uniprot ACC#: Q32ZI4
Transportation method: Shipped at Room temperature
Safety data sheet: Inquire at info@3hbiomedical.com