Urokinase, produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 419 amino acids (21-431) and having a molecular mass of 47.4 kDa (Molecular size on SDS-PAGE will appear at approximately 40-57 kDa). Urokinase is fused to 8 amino acid His-Tag at C-terminus and purified by proprietary chromatographic techniques.
Synonyms: PLAU, ATF, BDPLT5, QPD, u-PA, UPA, URK, Urokinase-type plasminogen activator, U-plasminogen activator, uPA, Urokinase-type plasminogen activator long chain A, Urokinase-type plasminogen activator short chain A, Urokinase-type plasminogen activator chain B.
Formulation: Urokinase protein solution (0.5mg/ml) containing Phosphate buffered saline (pH 7.4) and 10% glycerol.
Physical Appearance: Sterile Filtered clear solution.
Purity: Greater than 95% as determined by SDS-PAGE.
Source: Sf9, Baculovirus cells.
Amino acid sequence: SNELHQVPSN CDCLNGGTCV SNKYFSNIHW CNCPKKFGGQ HCEIDKSKTC YEGNGHFYRG KASTDTMGRP CLPWNSATVL QQTYHAHRSD ALQLGLGKHN YCRNPDNRRR PWCYVQVGLK PLVQECMVHD CADGKKPSSP PEELKFQCGQ KTLRPRFKII GGEFTTIENQ PWFAAIYRRH RGGSVTYVCG GSLISPCWVI SATHCFIDYP KKEDYIVYLG RSRLNSNTQG EMKFEVENLI LHKDYSADTL AHHNDIALLK IRSKEGRCAQ PSRTIQTICL PSMYNDPQFG TSCEITGFGK ENSTDYLYPE QLKMTVVKLI SHRECQQPHY YGSEVTTKML CAADPQWKTD SCQGDSGGPL VCSLQGRMTL TGIVSWGRGC ALKDKPGVYT RVSHFLPWIR SHTKEENGLA LLEHHHHHH.
Uniprot ACC number: P00749
Transportation method: Shipped with Ice Packs.
Stability: Store at +4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Safety data sheet: Inquire at
info@3hbiomedical.com