UBE2K produced in
E. coli is a single, non-glycosylated polypeptide chain containing 236 amino acids (1-200a. a.) and having a molecular mass of 26.5 kDa. UBE2K is fused to a 36 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Synonyms: HIP2, Huntingtin Interacting protein 2, HYPG, Ubiquitin-conjugating enzyme E2-25K kDa, Ubiquitin-protein ligase, Ubiquitin carrier protein, LIG, HIP-2, E2(25K), DKFZp686J24237, OTTHUMP00000218440, EC 6.3.2.19.
Formulation: The UBE2K protein solution (1mg/1ml) is formulated in 20mM Tris-HCl buffer (pH 7.5) 1mM DTT, 50mM NaCl, and 10% glycerol.
Physical Appearance: Sterile Filtered clear solution.
Purity: Greater than 95% as determined by: (a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Source:Escherichia coli.Amino acid sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMANI AVQRIKREFK EVLKSEETSK NQIKVDLVDE NFTELRGEIA GPPDTPYEGG RYQLEIKIPE TYPFNPPKVR FITKIWHPNI SSVTGAICLD ILKDQWAAAM TLRTVLLSLQ ALLAAAEPDD PQDAVVANQY KQNPEMFKQT ARLWAHVYAG APVSSPEYTK KIENLCAMGF DRNAVIVALS SKSWDVETAT ELLLSN.
Uniprot ACC number: P61086
Transportation method: Shipped with Ice Packs.
Stability: Store at +4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Please avoid freeze thaw cycles.
Safety data sheet: Inquire at
info@3hbiomedical.com