Ubiquitin-Conjugating Enzyme E2I, produced in
E. coli is a 19.5 kDa protein containing 171 amino acids. The UBE2I protein contains 6xHis tag and is purified by proprietary chromatographic techniques.
Synonyms: SUMO-conjugating enzyme UBC9, EC 6.3.2.-, SUMO-protein ligase, Ubiquitin-conjugating enzyme E2 I, Ubiquitin-protein ligase I, Ubiquitin carrier protein I, Ubiquitin carrier protein 9, p18, UBC9, C358B7.1.
Formulation: Lyophilized from a 0.2μm filtered concentrated (1mg/ml) solution in 1X PBS and 1mM DTT, pH 7.5.
Physical Appearance: Sterile Filtered white lyophilized powder.
Purity: Greater than 95% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Source:Escherichia coli.Amino acid sequence: MHHHHHHAMGTLNMSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKC
KFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFAPS.
Uniprot ACC number: P63279
Transportation method: Shipped at room temperature.
Stability: Lyophilized UBE2I although stable at room temperature. Erature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution UBE2I should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Solubility: It is recommended to reconstitute the lyophilized UBE2I in sterile water not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at
info@3hbiomedical.com