Tissue Plasminogen Activator, produced in CHO cells is a single, glycosylated polypeptide chain containing 527 amino acids and having a molecular mass of 59008.71 Dalton. tPA is a serine protease enzyme that converts plasminogen to plasmin. The tPA is purified by proprietary chromatographic techniques.
Synonyms: Tissue-type plasminogen activator, EC 3.4.21.68, tPA, t-PA, t-plasminogen activator, TPA, T-PA, DKFZp686I03148.
Formulation: Each mg of t-PA contains 1.7 gr L-arginine, 0.5 gr phosphoric acid and 4 mg tween 80.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 98% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Source: Chinese Hamster Ovary Cells (CHO)
Uniprot ACC number: P00750
Transportation method: Shipped at room temperature.
Stability: Lyophilized t-PA although stable at room temperature. Erature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution tPA should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Solubility: It is recommended to reconstitute the lyophilized t-PA in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
References: 1. Title:Thrombin-activable Fibrinolysis Inhibitor (TAFI) Zymogen Is an Active Carboxypeptidase. Publication:First Published on November 30, 2006, doi: 10.1074/jbc. M606559200 February 2, 2007 The Journal of Biological Chemistry, 282, 3066-3076. Link:http://www. Jbc. Org/content/282/5/3066. Full2. Title:Biochemical characterization of bovine plasma thrombin-activatable fibrinolysis inhibitor (TAFI). Publication:BMC Biochemistry 2009, 10:13 doi:10.1186/1471-2091-10-13. Link:http://www. Biomedcentral.com/1471-2091/10/13/
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