Thyroid Peroxidase, produced in SF9 is a glycosylated, polypeptide chain containing 834 amino acids and having a molecular mass of 92,872 Dalton (excluding glycosylation), 101 kDa total mass. The TPO is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.
Synonyms: Thyroid peroxidase, EC 1.11.1.8, TPO, MSA, TPX.
Formulation: TPO is supplied in 16mM HEPES pH-7.6, 160mM NaCl, 0.08mM Kl and 20% glycerol.
Purity: Greater than 95% as determined by SDS-PAGE.
Source: Sf9 insect cells.
Uniprot ACC number: P07202
Transportation method: Shipped with Ice Packs.
Stability: Store at +4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.
Coating concentration: 0.15-0.375 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.
Immunological functions: 1. Binds IgG-type human auto-antibodies.2. Auto-antibodies to TPO recognize conformation-dependent epitopes.3. Standard ELISA test (checker-board analysis of positive/negative sera panels, including international reference serum NIBSC 66/387).
Safety data sheet: Inquire at
info@3hbiomedical.com