SORD, produced in
E. coli is a single, non-glycosylated, polypeptide chain containing 377 amino acids (1-357 a. a.) and having a molecular mass of 40.4 kDa. SORD protein is fused to a 20 amino acid His tag at N-terminus and is purified by standard chromatography.
Synonyms: EC 1.1.1.14, SORD1,SORD, L-iditol 2-dehydrogenase, DHSO, Sorbitol Dehydrogenase.
Formulation: SORD protein solution (0.5mg/ml) is formulated in 20mM Tris-HCl pH 8, 0.2M NaCl, 5mM DTT & 20% glycerol.
Physical Appearance: Sterile filtered colorless solution.
Purity: Greater than 90% as determined by SDS-PAGE.
Source:Escherichia coli.Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MAAAAKPNNL SLVVHGPGDL RLENYPIPEP GPNEVLLRMH SVGICGSDVH YWEYGRIGNF IVKKPMVLGH EASGTVEKVG SSVKHLKPGD RVAIEPGAPR ENDEFCKMGR YNLSPSIFFC ATPPDDGNLC RFYKHNAAFC YKLPDNVTFE EGALIEPLSV GIHACRRGGV TLGHKVLVCG AGPIGMVTLL VAKAMGAAQV VVTDLSATRL SKAKEIGADL VLQISKESPQ EIARKVEGQL GCKPEVTIEC TGAEASIQAG IYATRSGGTL VLVGLGSEMT TVPLLHAAIR EVDIKGVFRY CNTWPVAISM LASKSVNVKP LVTHRFPLEK ALEAFETFKK GLGLKIMLKC DPSDQNP.
Uniprot ACC number: Q00796
Transportation method: Shipped with Ice Packs.
Stability: Store at +4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Biological Activity: Specific activity is > 0.2 units/mg, in which one unit will convert 1.0 umole of D-fructose to D-sorbitol per minute at pH 7.5 at 25°C.
Safety data sheet: Inquire at
info@3hbiomedical.com