Secreted Phospholipase A2-IIA, is manufactured with N-terminal fusion of HisTag. PLA2G2A His-Tagged Fusion Protein is 15.8 kDa containing 124 amino acid residues of the human secreted phospholipase A2-IIA and 16 additional amino acid residues – HisTag.
Synonyms: MOM1, PLA2, PLA2B, PLA2L, PLA2S, PLAS1, sPLA2, Phospholipase A2 membrane associated, EC 3.1.1.4, Phosphatidylcholine 2-acylhydrolase, Group IIA phospholipase A2, GIIC sPLA2, Non-pancreatic secretory phospholipase A2, NPS-PLA2, sPLA2-IIA, PLA2G2A.
Formulation: Lyophilized from 0.5 mg/ml in 0.05M Acetate buffer pH 4.
Physical Appearance: Filtered lyophilized (freeze-dried) powder.
Purity: Greater than 95% as determined by SDS-PAGE.
Source:Escherichia coli.Amino acid sequence: MRGSHHHHHH GMASHMNLVN FHRMIKLTTG KEAALSYGFY GCHCGVGGRG SPKDATDRCC VTHDCCYKRL EKRGCGTKFL SYKFSNSGSR ITCAKQDSCR SQLCECDKAA ATCFARNKTT YNKKYQYYSN KHCRGSTPRC.
Uniprot ACC number: P14555
Transportation method: Shipped at room temperature.
Stability: Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at +4°C for a limited period of time; it does not show any change after two weeks at +4°C.
Solubility: It is recommended to add 0.1M Acetate buffer pH 4 to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. For conversion into higher pH value, an intensive dilution by relevant buffer to a concentration of 10μg/ml is recomended. In higher concentrations the solubility of the PLA2G2A antigen is limited. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Safety data sheet: Inquire at
info@3hbiomedical.com