PDHX, produced in
E. coli is a single, non-glycosylated polypeptide chain containing 471 amino acids (54-501 a. a.) and having a molecular mass of 50.4 kDa. PDHX is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Synonyms: DLDBP, E3BP, OPDX, PDX1, proX, Pyruvate Dehydrogenase Complex, Component X, Dihydrolipoamide Dehydrogenase-Binding Protein Of Pyruvate Dehydrogenase Complex, Lipoyl-Containing Pyruvate Dehydrogenase Complex Component X, Pyruvate Dehydrogenase Complex, Lipoyl-Containing Component X, Pyruvate Dehydrogenase Complex, E3-Binding Protein Subunit, Pyruvate Dehydrogenase Protein X Component, Mitochondrial, E3-Binding Protein.
Formulation: PDHX protein solution (0.25mg/ml) containing Phosphate buffer saline (pH 7.4) and 20% glycerol, 1mM DTT.
Physical Appearance: Sterile Filtered clear solution.
Purity: Greater than 85% as determined by SDS-PAGE.
Source:Escherichia coli.Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MGSGDPIKIL MPSLSPTMEE GNIVKWLKKE GEAVSAGDAL CEIETDKAVV TLDASDDGIL AKIVVEEGSK NIRLGSLIGL IVEEGEDWKH VEIPKDVGPP PPVSKPSEPR PSPEPQISIP VKKEHIPGTL RFRLSPAARN ILEKHSLDAS QGTATGPRGI FTKEDALKLV QLKQTGKITE SRPTPAPTAT PTAPSPLQAT AGPSYPRPVI PPVSTPGQPN AVGTFTEIPA SNIRRVIAKR LTESKSTVPH AYATADCDLG AVLKVRQDLV KDDIKVSVND FIIKAAAVTL KQMPDVNVSW DGEGPKQLPF IDISVAVATD KGLLTPIIKD AAAKGIQEIA DSVKALSKKA RDGKLLPEEY QGGSFSISNL GMFGIDEFTA VINPPQACIL AVGRFRPVLK LTEDEEGNAK LQQRQLITVT MSSDSRVVDD ELATRFLKSF KANLENPIRL A.
Uniprot ACC number: O00330
Transportation method: Shipped with Ice Packs.
Stability: Store at +4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Safety data sheet: Inquire at
info@3hbiomedical.com