PNPO, fused with a 21 amino acid His tag at N-terminus produced in
E. coli is a single, non-glycosylated, polypeptide chain containing 226 amino acids (57-261 a. a.) and having a molecular mass of 25.9 kDa. The PNPO is purified by proprietary chromatographic techniques.
Synonyms: Pyridoxine-5'-phosphate oxidase, Pyridoxamine-phosphate oxidase, PNPO, PDXPO, FLJ10535.
Formulation: The PNPO solution (0.5 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 10% glycerol, 0.1M NaCl and 0.1mM PMSF.
Physical Appearance: Sterile Filtered colorless solution.
Purity: Greater than 90% as determined by SDS-PAGE.
Source:Escherichia coli.Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MDPVKQFAAW FEEAVQCPDI GEANAMCLAT CTRDGKPSAR MLLLKGFGKD GFRFFTNFES RKGKELDSNP FASLVFYWEP LNRQVRVEGP VKKLPEEEAE CYFHSRPKSS QIGAVVSHQS SVIPDREYLR KKNEELEQLY QDQEVPKPKS WGGYVLYPQV MEFWQGQTNR LHDRIVFRRG LPTGDSPLGP MTHRGEEDWL YERLAP.
Uniprot ACC number: Q9NVS9
Transportation method: Shipped with Ice Packs.
Stability: Store at +4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Safety data sheet: Inquire at
info@3hbiomedical.com