PPP1CA, produced in
E. coli is a single, non-glycosylated polypeptide chain containing 350 amino acids (1-330 a. a.) and having a molecular mass of 39.7 kDa. PPP1CA is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Synonyms: Serine/threonine-protein phosphatase PP1-alpha catalytic subunit, PP-1A, PPP1CA, PPP1A, PP1alpha.
Formulation: PPP1CA protein solution (0.2mg/ml) containing 50mM Tris-HCl (pH 8.5),0.2M NaCl, 1mM DTT, 0.1mM PMSF, 1mM MnCl2 and 50% glycerol.
Physical Appearance: Sterile filtered yellowish solution.
Purity: Greater than 80.0% as determined by SDS-PAGE.
Source:Escherichia coli.Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MSDSEKLNLD SIIGRLLEVQ GSRPGKNVQL TENEIRGLCL KSREIFLSQP ILLELEAPLK ICGDIHGQYY DLLRLFEYGG FPPESNYLFL GDYVDRGKQS LETICLLLAY KIKYPENFFL LRGNHECASI NRIYGFYDEC KRRYNIKLWK TFTDCFNCLP IAAIVDEKIF CCHGGLSPDL QSMEQIRRIM RPTDVPDQGL LCDLLWSDPD KDVQGWGEND RGVSFTFGAE VVAKFLHKHD LDLICRAHQV VEDGYEFFAK RQLVTLFSAP NYCGEFDNAG AMMSVDETLM CSFQILKPAD KNKGKYGQFS GLNPGGRPIT PPRNSAKAKK.
Uniprot ACC number: P62136
Transportation method: Shipped with Ice Packs.
Stability: Store at +4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Biological Activity: Specific activity: >3,000 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37C, pH 7.5 using 30mM of substrate.
Safety data sheet: Inquire at
info@3hbiomedical.com