PDI, produced in
E. coli is a single, non-glycosylated, polypeptide chain containing 503 amino acids and having a molecular mass of 62.4 kDa. The PDI is fused to a 12 amino acid His tag (515 a. a. total) at N-terminal and purified by proprietary chromatographic techniques.
Synonyms: Protein Disulfide Isomerase, PDI, EC 5.3.4.1, Prolyl 4-hydroxylase subunit beta, Cellular thyroid hormone-binding protein, p55, P4HB, ERBA2L, PDIA1, PO4DB, DSI, GIT, PHDB, PO4HB, PROHB, P4Hbeta.
Formulation: The PDI protein (1mg/ml)solution was lyophilized from PBS pH-7.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as determined by:a) Analysis by RP-HPLC. B) Analysis by SDS-PAGE.
Source:Escherichia coli.Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Arg-Arg-Ala.
Uniprot ACC number: P07237
Transportation method: Shipped at room temperature.
Stability: Lyophilized Protein Disulfide Isomerase although stable at room temperature. Erature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Human PDI should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Solubility: It is recommended to reconstitute the lyophilized PDI in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
References: Title: Protein disulfide isomerases are antibody targets during immune-mediated tumor destructionPublications: Blood 113.8 (2009): 1681-1688. Link: http://www. Ncbi. Nlm. Nih. Gov/pmc/articles/PMC2647666/
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