PSMF1, produced in
E. coli is a single, non-glycosylated polypeptide chain containing 291 amino acids (1-271) and having a molecular mass of 31.9 kDa. PSMF1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Synonyms: Proteasome inhibitor PI31 subunit, hPI31, PSMF1, PI31.
Formulation: The PSMF1 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 20% glycerol, 1mM DTT and 0.1mM PMSF.
Physical Appearance: Sterile Filtered colorless solution.
Purity: Greater than 85% as determined by SDS-PAGE.
Source:Escherichia coli.Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MAGLEVLFAS AAPAITCRQD ALVCFLHWEV VTHGYCGLGV GDQPGPNDKK SELLPAGWNN NKDLYVLRYE YKDGSRKLLV KAITVESSMI LNVLEYGSQQ VADLTLNLDD YIDAEHLGDF HRTYKNSEEL RSRIVSGIIT PIHEQWEKAN VSSPHREFPP ATAREVDPLR IPPHHPHTSR QPPWCDPLGP FVVGGEDLDP FGPRRGGMIV DPLRSGFPRA LIDPSSGLPN RLPPGAVPPG ARFDPFGPIG TSPPGPNPDH LPPPGYDDMY L.
Uniprot ACC number: Q92530
Transportation method: Shipped with Ice Packs.
Stability: Store at +4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Safety data sheet: Inquire at
info@3hbiomedical.com