POLR2J2, produced in
E. coli is a single, non-glycosylated polypeptide chain containing 138 amino acids (1-115) and having a molecular mass of 15.5 kDa. POLR2J2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Synonyms: HRPB11B, RPB11b1, POLR2J2, DNA-directed RNA polymerase II subunit RPB11-b1.
Formulation: The POLR2J2 solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol, 1mM DTT and 250mM Imidazole.
Physical Appearance: Sterile Filtered colorless solution.
Purity: Greater than 95% as determined by SDS-PAGE.
Source:E. coli.
Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MGSMNAPPAF ESFLLFEGEK ITINKDTKVP KACLFTINKE DHTLGNIIKS QLLKDPQVLF AGYKVPHPLE HKIIIRVQTT PDYSPQEAFT NAITDLISEL SLLEERFRTC LLPLRLLP.
Uniprot ACC number: F6W009
Transportation method: Shipped with Ice Packs.
Stability: Store at +4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Safety data sheet: Inquire at
info@3hbiomedical.com