POLR2F, produced in
E. coli is a single, non-glycosylated polypeptide chain containing 150 amino acids (1-127) and having a molecular mass of 16.9 kDa (Molecular size on SDS-PAGE will appear higher). POLR2F is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Synonyms: Polymerase (RNA) II (DNA Directed) Polypeptide F, DNA Directed RNA Polymerase II 14.4 Kda Polypeptide, POLRF, RPABC14.4, RPB14.4, RPC15, DNA-Directed RNA Polymerase II Subunit F, DNA-Directed RNA Polymerases I, II, And III 14.4 KDa Polypeptide, RNA Polymerases I, II, And III Subunit ABC2, RPB6 Homolog, HRBP14.4, RPABC2, RPB6, DNA-Directed RNA Polymerases I, II, And III Subunit RPABC2, RNA Polymerase II Subunit 14.4 KD, POLR2F.
Formulation: The POLR2F solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 20% glycerol and 1mM DTT.
Physical Appearance: Sterile Filtered colorless solution.
Purity: Greater than 95% as determined by SDS-PAGE.
Source:E. coli.
Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MGSMSDNEDN FDGDDFDDVE EDEGLDDLEN AEEEGQENVE ILPSGERPQA NQKRITTPYM TKYERARVLG TRALQIAMCA PVMVELEGET DPLLIAMKEL KARKIPIIIR RYLPDGSYED WGVDELIITD.
Uniprot ACC number: P61218
Transportation method: Shipped with Ice Packs.
Stability: Store at +4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Safety data sheet: Inquire at
info@3hbiomedical.com