MMP-2, produced in HEK293 cells is a proform of the Human MMP-2 (Ala30-Cys660) and fused with a ployhistide tag at the C-terminus, having an Mw of 71 kDa. MMP-2 is purified by proprietary chromatographic techniques.
Synonyms: 72 kDa type IV collagenase, 72 kDa gelatinase, Gelatinase A, Matrix metalloproteinase-2, MMP-2, TBE-1, MMP2, CLG4A, CLG4, MONA, MMP-II.
Formulation: The MMP-2 is supplied as a 0.2µm filtered solution in 20mM Tris-HCl, 150mM NaCl and 0.05% Brij 35, pH 7.4.
Physical Appearance: The MMP-2 is supplied as a sterile Filtered colorless solution.
Purity: Greater than 95% as determined by SDS-PAGE.
Source: HEK293 cells.
Uniprot ACC number: P08253
Transportation method: Shipped with Ice Packs.
Stability: Store MMP-2 at +4°C if entire vial will be used within 2-4 weeks. Store frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.
Biological Activity: The activity was measured by its ability to cleave fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2 (RND,Catalog # ES001)., The specific activity is > 1,000 pmoles/min/µg. Recombinant Human MMP-2 protein pro form needs to be activated with p-aminophenylmercuric acetate (APMA). Activation Protocol:1. Dilute MMP2 to 100µg/ml in the Assay Buffer: 50mM Tris, 10mM CaCl2, 150mM NaCl, 0.05% (w/v) and Brij 35, pH 7.5.2. Activate MMP2 by adding APMA to a final concentration of 1mM. (Sigma, Catalog # A9563) and 100mM stock in DMSO.3. Incubate at 37°C for 1 hour.
References: Title:MMP-2 regulates human platelet activation by interacting withintegrin aIIbb 3. Publication:To cite this article: Choi W-S, Jeon O-H, Kim H-H, Kim D-S. MMP-2 regulates human platelet activation by interacting with integrin aIIbb3. J Thromb Haemost 2008; 6: 517–23. Link:http://onlinelibrary.wiley.com/doi/10.1111/j.1538-7836.2007.02871. X/pdf
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