MMP-1, produced in HEK293 cells is a proform of the Human MMP1 (Met1-Asn469) and fused with a ployhistide tag at the C-terminus, having an Mw of 52 kDa. MMP-1 is purified by proprietary chromatographic techniques.
Synonyms: Interstitial collagenase, Fibroblast collagenase, Matrix metalloproteinase-1, MMP-1, MMP1, CLG, CLGN.
Formulation: The MMP-1 is supplied as a 0.2µm filtered solution in MES, NaCl, Glycerol and Brij35.
Physical Appearance: Sterile Filtered colorless solution.
Purity: Greater than 95% as determined by SDS-PAGE.
Source: HEK293 cells.
Uniprot ACC number: P03956
Transportation method: Shipped with Ice Packs.
Stability: Store at +4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.
Biological Activity: The activity was measured by its ability to cleave fluorogenic peptide substrate, Mca-KPLGL-Dpa-AR-NH2, The specific activity is > 400 pmoles/min/µg. Recombinant Human MMP-1 protein pro form needs to be activated with p-aminophenylmercuric acetate (APMA). Activation Protocol:1. Dilute MMP1 to 50µg/ml in the Assay Buffer: 50mM Tris, 10mM CaCl2, 150mM NaCl, 0.05% (w/v) and Brij 35, pH 7.5.2. Activate MMP1 by adding APMA to a final concentration of 1mM. (Sigma, Catalog # A9563) and 100mM stock in DMSO.3. Incubate at 37°C for 2 hours.
Safety data sheet: Inquire at
info@3hbiomedical.com