LIPG, produced in
E. coli is a single, non-glycosylated polypeptide chain containing 343 amino acids (21-340) and having a molecular mass of 38 kDa. LIPG is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Synonyms: LIPG, Lipase Endothelial, EDL, EL, Endothelial Cell-Derived Lipase, EC 3.1.1.3, PRO719, Endothelial Lipase, Lipoprotein Lipase H, EC 3.1.1.
Formulation: The LIPG solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 10% glycerol and 0.4M Urea.
Physical Appearance: Sterile Filtered clear solution.
Purity: Greater than 85% as determined by SDS-PAGE.
Source:Escherichia coli.Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MGSSPVPFGP EGRLEDKLHK PKATQTEVKP SVRFNLRTSK DPEHEGCYLS VGHSQPLEDC SFNMTAKTFF IIHGWTMSGI FENWLHKLVS ALHTREKDAN VVVVDWLPLA HQLYTDAVNN TRVVGHSIAR MLDWLQEKDD FSLGNVHLIG YSLGAHVAGY AGNFVKGTVG RITGLDPAGP MFEGADIHKR LSPDDADFVD VLHTYTRSFG LSIGIQMPVG HIDIYPNGGD FQPGCGLNDV LGSIAYGTIT EVVKCEHERA VHLFVDSLVN QDKPSFAFQC TDSNRFKKGI CLSCRKNRCN SIGYNAKKMR NKRNSKMYLK TRA.
Uniprot ACC number: Q9Y5X9
Transportation method: Shipped with Ice Packs.
Stability: Store at +4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Safety data sheet: Inquire at
info@3hbiomedical.com