GZMB produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 235 amino acids (19-247a. a.) and having a molecular mass of 26.5 kDa. (Molecular size on SDS-PAGE will appear at approximately 28-40 kDa). GZMB is expressed with an 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Synonyms: Granzyme B, T-Cell Serine Protease 1-3E, Cathepsin G-Like 1, Granzyme B (Granzyme 2, Cytotoxic T-Lymphocyte-Associated Serine Esterase 1, Cytotoxic T-Lymphocyte Proteinase 2, Cytotoxic Serine Protease B , Human Lymphocyte Protein, Fragmentin 2, EC 3.4.21.79, CTSGL1, CTLA1 , CSPB , CGL1, SECT , C11, HLP , Cytotoxic T-Lymphocyte-Associated Serine Esterase 1, Lymphocyte Protease, Fragmentin-2 , Granzyme 2 , Granzyme-2 , EC 3.4.21, CTLA-1 , CGL-1, CSP-B, CCPI , GRB.
Formulation: GZMB protein solution (0.5mg/ml) contains Phosphate Buffered Saline (pH 7.4) ,20% glycerol and 1mM DTT.
Physical Appearance: Sterile Filtered colorless solution.
Purity: Greater than 95% as determined by SDS-PAGE.
Source: Sf9, Baculovirus cells.
Amino acid sequence: GEIIGGHEAK PHSRPYMAYL MIWDQKSLKR CGGFLIRDDF VLTAAHCWGS SINVTLGAHN IKEQEPTQQF IPVKRPIPHP AYNPKNFSND IMLLQLERKA KRTRAVQPLR LPSNKAQVKP GQTCSVAGWG QTAPLGKHSH TLQEVKMTVQ EDRKCESDLR HYYDSTIELC VGDPEIKKTS FKGDSGGPLV CNKVAQGIVS YGRNNGMPPR ACTKVSSFVH WIKKTMKRYH HHHHH
Uniprot ACC number: P10144
Transportation method: Shipped with Ice Packs.
Stability: Store at +4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Biological Activity: Specific activity is > 7,000 pmol/min/ug, and is defined as the amount of enzyme that cleave 1pmole of Boc-Ala-Ala-Asp-SBzl at 37C.
Safety data sheet: Inquire at
info@3hbiomedical.com