GMDS, produced in
E. coli is a single, non-glycosylated polypeptide chain containing 392 amino acids (1-372) and having a molecular mass of 44.1 kDa. GMDS is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Synonyms: GDP-mannose 4,6-dehydratase, GMD, SDR3E1, short chain dehydrogenase/reductase family 3E member 1, GDP-D-mannose dehydratase, EC 4.2.1.47.
Formulation: The GMDS solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 0.1M NaCl, 0.1mM PMSF and 30% glycerol.
Physical Appearance: Sterile Filtered colorless solution.
Purity: Greater than 95% as determined by SDS-PAGE.
Source:E. coli.
Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MAHAPARCPS ARGSGDGEMG KPRNVALITG ITGQDGSYLA EFLLEKGYEV HGIVRRSSSF NTGRIEHLYK NPQAHIEGNM KLHYGDLTDS TCLVKIINEV KPTEIYNLGA QSHVKISFDL AEYTADVDGV GTLRLLDAVK TCGLINSVKF YQASTSELYG KVQEIPQKET TPFYPRSPYG AAKLYAYWIV VNFREAYNLF AVNGILFNHE SPRRGANFVT RKISRSVAKI YLGQLECFSL GNLDAKRDWG HAKDYVEAMW LMLQNDEPED FVIATGEVHS VREFVEKSFL HIGKTIVWEG KNENEVGRCK ETGKVHVTVD LKYYRPTEVD FLQGDCTKAK QKLNWKPRVA FDELVREMVH ADVELMRTNP NA.
Uniprot ACC number: O60547
Transportation method: Shipped with Ice Packs.
Stability: Store at +4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Safety data sheet: Inquire at
info@3hbiomedical.com