DLST is a Full-length cDNA coding for the mature form of the human OGDC-E2 protein having a molecular mass of 42,301 Dalton (pH 6.3). DLST protein is fused to a hexa-histidine purification tag.
Synonyms: dihydrolipoamide S-succinyltransferase (E2 component of 2-oxo-glutarate complex), Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex, component E2, DLST, E2K, OGDC-E2, EC 2.3.1.61, EC 2.3.1.
Formulation: DLST (0.74mg/ml) is supplied in 20mM HEPES buffer pH 8.0, 200mM NaCl and 20% glycerol.
Purity: DLST purity was found to be greater than 75% as determined by SDS-PAGE.
Source: Sf9 insect cells.
Uniprot ACC number: P36957
Transportation method: Shipped with Ice Packs.
Stability: Store DLST at +4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.
Applications: Western blot with anti-M2-Antigen autoantibody-positive patient sera or monoclonal anti-hexa-His-tag antibody.
Coating concentration: 0.4-1.0 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.
Immunological functions: 1. DLST binds IgG-type human auto-antibodies. 2. Standard ELISA test (checkerboard analysis of positive/negative sera panels); immunodot test with positive/negative sera panels.
Safety data sheet: Inquire at
info@3hbiomedical.com