Deoxyribonuclease I, produced in CHO is a glycosylated, polypeptide chain containing 260 amino acids and having a total molecular mass of 37,000 Dalton with a molecular formula of C1321H1999N339O396S9. DNase is purified by proprietary chromatographic techniques.
Synonyms: EC 3.1.21.1, Deoxyribonuclease I, DNase I, DNL1, DRNI, FLJ38093, DNASE1.
Formulation: Each mg contains 150 µg calcium chloride dehydrate and 8.77 mg NaCl.
Physical Appearance: Sterile liquid colorless solution at a concentration of 1mg/ml.
Source: Chinese Hamster Ovary Cells.
Uniprot ACC number: P24855
Transportation method: Shipped with Ice Packs.
Stability: 2 years when stored at +4°C, three weeks at 15°C, pH-6.3.
Specific Activity: 1000IU/1mg.
Unit Definition: Dnase is generally assayed according to the photometric method developed by Kunitz. One Dnase unit results in an increase in absorbance at 260nm of 0.001/minute at 25°C when acting upon highly polymerized solution of DNA at pH-5. Also 0.005 Kunitz unit digests 1µgof lambda phage DNA in 10 minutes at 37°C in50mM Tris, 1mMMg++, pH 7.8 in a 50ul reaction.
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