Cytochrome P450 2D6, (also called liver/kidney microsomal antigen 1) produced in SF9, is a glycosylated, polypeptide chain having a molecular mass of 60 kDa. The LKM1 is purified by proprietary chromatographic techniques.
Synonyms: Cytochrome P450 2D6, CYPIID6, P450-DB1, Debrisoquine 4-hydroxylase, CYP2D6, CPD6, CYP2D, CYP2D@, CYP2DL1, P450C2D, MGC120389, MGC120390, LKM1, liver/kidney microsomal antigen 1.
Formulation: LKM-1 is supplied in 17mM HEPES pH-7.5, 425mM NaCl, 1mM DTT, 1% Triton X-100 and 15% glycerol.
Physical Appearance: Sterile filtered liquid formulation.
Purity: Greater than 95% as determined by SDS-PAGE.
Source: Sf9 insect cells.
Uniprot ACC number: P10635
Transportation method: Shipped with Ice Packs.
Stability: Store at +4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.
Applications: Western-Blot with monoclonal anti-LKM-1 antibody and anti-LKM-1 autoantibody- positive sample.
Coating concentration: 0.35-0.6 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for labeling of functional groups.
Immunological functions: 1. Binds IgG-type human auto-antibodies.2. Standard ELISA test (checker-board analysis of positive/negative samples). 3. Immunodot analysis with positive/negative samples.
Safety data sheet: Inquire at
info@3hbiomedical.com