Recombinant Human Carbonic anhydrase 1 produced in
E. coli is a single, non-glycosylated polypeptide chain containing 281 amino acids (1-261 a. a.) and having a molecular mass of 31 kDa. Carbonic anhydrase 1 is fused to a 20 amino acids His-Tag at N-terminus and purified by conventional chromatography techniques.
Synonyms: CA-1, CA1, CAI, CA-I, Carbonate dehydratase I, Carbonic anhydrase I, Carbonic anhydrase 1, Car1.
Formulation: The Carbonic Anhydrase-1 protein solution contains 20mM Tris-HCl, pH 8, 1mM DTT and 10% Glycerol.
Physical Appearance: Sterile filtered colorless solution.
Purity: Greater than 95% as determined by SDS-PAGE.
Source:Escherichia coli.Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MASPDWGYDD KNGPEQWSKL YPIANGNNQS PVDIKTSETK HDTSLKPISV SYNPATAKEI INVGHSFHVN FEDNDNRSVL KGGPFSDSYR LFQFHFHWGS TNEHGSEHTV DGVKYSAELH VAHWNSAKYS SLAEAASKAD GLAVIGVLMK VGEANPKLQK VLDALQAIKT KGKRAPFTNF DPSTLLPSSL DFWTYPGSLT HPPLYESVTW IICKESISVS SEQLAQFRSL LSNVEGDNAV PMQHNNRPTQ PLKGRTVRAS F.
Uniprot ACC number: P00915
Transportation method: Shipped with Ice Packs.
Stability: Store at +4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Safety data sheet: Inquire at
info@3hbiomedical.com