Unit Definition: One unit of ClearCut DNase is defined as the amount of enzyme required to increase the absorbance at 260 nm by 0.001 per minute at +25°C and pH 5.0, using salmon sperm DNA as the substrate, according to the Kunitz assay method.
Storage Buffer: ClearCut DNase is supplied in a storage buffer containing 20 mM Tris-HCl (pH 7.5 at +25°C), 2 mM MgCl₂, 10 mM NaCl, 0.01% (v/v) Tween 20, and 50% (v/v) glycerol.
Quality Control: RNase activity was evaluated by gel electrophoresis after incubation of 2 μg of total eukaryotic RNA with 2U of enzyme in a 20 μL reaction volume for 1 hour at +37°C. No detectable RNase activity was observed.
Product Use: ccDNase is for research use only. It is not approved for human or animal use, or for application in clinical or in vitro diagnostic procedures. DNase activity is sensitive to metal chelating agents, transition metal ions (e.g., Zn²⁺) at millimolar concentrations, reducing compounds such as DTT, elevated salt concentrations, and trace levels of ionic detergents such as SDS (>0.1%). Heat inactivation can be achieved by incubation at +60°C for 5 min in the presence of 5
mM DTT.
Shipping and Storage: The product is shipped on ice packs. Upon receipt, store ClearCut DNase (MB6318a) at -20oC in a manual defrost freezer. Store 10x DNase Enzyme Buffer (Cat. No. MB6038b)
at +4°C, or alternatively store it at −20°C together with ClearCut DNase.
Application Example: Following in vitro transcription using ScienCell’s TranscriEase T7 RNA Synthesis Kit, 1 μL of ClearCut DNase was added directly to the reaction mixture (containing 1 μg DNA template and up to 50 μg RNA product) and incubated at +37°C for 10 minutes. Removal of up to 99 % of template DNA was confirmed by qPCR analysis.