Recombinant human Visfatin produced in
E. coli is a single, non-glycosylated, polypeptide chain containing 466 amino acids. The total molecular mass is 52.6 kDa (calculated). The Visfatin is purified by Flag-affinity chromatography.
Synonyms: PBEF, Pre-B cell colony-enhancing factor, Nicotinamide pH ospH oribosyltransferase NAmPRTase, Nampt, MGC117256, DKFZP666B131, 1110035O14Rik.
Formulation: Visfatin was Lyophilized with no additives.
Physical Appearance: Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 90% as determined by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: MPPNTSKVYS YFECREKKTE NSKLRKVKYE ETVFYGLQYI LNKYLKGKVV TKEKIQEAKD VYKEHFQDDV FNEKGWNYIL EKYDGHLPIE IKAVPEGFVI PRGNVLFTVE NTDPECYWLT NWIETILVQS WYPITVATNS REQKKILAKY LLETSGNLDG LEYKLHDFGY RGVSSQETAG IGASAHLVNF KGTDTVAGLA LIKKYYGTKD PVPGYSVPAA EHSTITAWGK DHEKDAFEHI VTQFSSVPVS VVSDSYDIYN ACEKIWGEDL RHLIVSRSTQ APLIIRPDSG NPLDTVLKVL EILGKKFPVT ENSKGYKLLP PYLRVIQGDG VDINTLQEIV EGMKQKMWSI ENIAFGSGGG LLQKLTRDLL NCSFKCSYVV TNGLGINVFK DPVADPNKRS KKGRLSLHRT PAGNFVTLEE GKGDLEEYGQ DLLHTVFKNG KVTKSYSFDE IRKNAQLNIE LEAAHH.
Uniprot ACC number: P43490
Transportation method: Shipped at room temperature.
Stability: Lyophilized Visfatin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Visfatin should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The activity is determined by its ability to induce IL-6, IL-1 beta and TNF Alpha production from human PBMCs at 100 ng/ml.
Solubility: Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with 20 mM HCl at a concentration of 0.1 mg/mL, which can be further diluted into other aqueous solutions. Wait several minutes for full reconstitution and solubility.
Safety data sheet: Inquire at info@3hbiomedical.com