Recombinant Human VEGF 121 amino acids, produced in HEK cells is a glycosylated 37 kDa homodimer and 50 kDa homotrimer. The VEGF is purified by proprietary chromatographic techniques.
Synonyms: Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, MGC70609.
Formulation: VEGF was lyophilized from a 0.2 µm filtered solution containing 1xPBS.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as obsereved by SDS-PAGE.
Source: HEK.
Uniprot ACC number: P15692
Transportation method: Shipped at room temperature.
Stability: Lyophilized VEGF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution VEGF should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The specific activity was determined by the dose-dependent stimulation of the proliferation of HUVEC cells (Human Umbilical Vein Endothelial Cells), the ED50 is 3 ng/ml.
Solubility: It is recommended to reconstitute the lyophilized VEGF in sterile PBS containing 0.1% endotoxin-free recombinant HSA.
Safety data sheet: Inquire at info@3hbiomedical.com