Recombinant human Vascular Endothelial Growth Factor produced in
E. coli is a double, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 38.2 kDa. The VEGF is purified by proprietary chromatographic techniques.
Synonyms: Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, MGC70609.
Formulation: The VEGF protein was lyophilized from a concentrated (1mg/ml) solution with no additives.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKpH QGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR
Uniprot ACC number: P15692
Transportation method: Shipped at room temperature.
Stability: Lyophilized VEGF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution VEGF should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 3.7-5.6 ng/ml
, corresponding to a Specific Activity of 178, 570-270, 270IU/mg.
Solubility: It is recommended to reconstitute the lyophilized VEGF in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
References: 1. Title:Expression of VEGF and Its Receptors in the Bovine Endometrium Throughout the Estrous Cycle: Effects of VEGF on Prostaglandin Production in Endometrial Cells. Publication:Journal of Reproduction and DevelopmentVol.56 (2010) No.2 April P 223-229 \Link:https://www. jstage. jst. go. jp/article/jrd/56/2/56_09-139S/_article 2. Title: Cardio-specific long-term gene expression in a porcine model after selective pressure-regulated retroinfusion of adeno-associated viral (AAV) vectorsPublications: Gene therapy 15.1 (2008): 12-17. Link: http://www. nature. com/gt/journal/v15/n1/full/3303035a. html3. Title: SU11248, A selective tyrosine kinases inhibitor suppresses breast tumor angiogenesis and growth via targeting both tumor vasculature and breast cancer cellsPublication: Cancer biology & therapy 10.7 (2010): 703-711. Link: http://www. tandfonline. com/doi/full/10.4161/cbt.10.7.12904
Safety data sheet: Inquire at info@3hbiomedical.com