Recombinant human Uteroglobin produced in
E. coli is a single, non-glycosylated polypeptide chain containing 70 amino acids and having a molecular mass of 15.8 kDa. The SCGB1A1 is purified by proprietary chromatographic techniques.
Synonyms: Uteroglobin, Clara cell pH ospH olipid-binding protein, CCPBP, Clara cells 10 kDa secretory protein, CC10, Secretoglobin family 1A member 1, Urinary protein 1, UP-1, UP1, Urine protein 1, SCGB1A1, CCSP, UGB, CC16.
Formulation: The Uteroglobin protein was lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 98% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: EICPSFQRVI ETLLMDTPSS YEAAMELFSP DQDMREAGAQ LKKLVDTLPQ KPRESIIKLM EKIAQSSLCN.
Uniprot ACC number: P11684
Transportation method: Shipped at room temperature.
Stability: Lyophilized Uteroglobin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution SCGB1A1 should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The ED50 as determined by the ability of the i mMobilized protein to support the adhesion of the A549 human lung carcinoma cells is less than 5.0 µg/ml, corresponding to a specific activity of > 200 IU/mg.
Solubility: It is recommended to reconstitute the lyophilized SCGB1A1 in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com