Recombinant human TNFR2 Human produced in
E. coli is a single, non-glycosylated polypeptide chain containing 184 amino acids and having a molecular mass of 20 kDa. The TNFR2 is purified by proprietary chromatographic techniques.
Synonyms: Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor 2, TNF-R2, Tumor necrosis factor receptor type II, TNF-RII, TNFR-II, p75, p80 TNF-Alpha receptor, TNFRSF1B, TNFBR, TNFR2.
Formulation: TNFR2 was lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 97% as determined by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: MPAQVAFTPY APEPGSTCRL REYYDQTAQM CCSKCSPGQH AKVFCTKTSD TVCDSCEDST YTQLWNWVPE CLSCGSRCSS DQVETQACTR EQNRICTCRP GWYCALSKQE GCRLCAPLRK CRPGFGVARP GTETSDVVCK PCAPGTFSNT TSSTDICRpH QICNVVAIPG NASMDAVCTS TSPT.
Uniprot ACC number: P20333
Transportation method: Shipped at room temperature.
Stability: Lyophilized TNFR2 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution TNFR2 should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The ED50 as determined by its ability to inhibit the TNF-a mediated cytotoxicity in the L-929 cells is less than 0.2 µg/ml, corresponding to a specific activity of > 5000 IU/mg in the presence of 0.25 ng/ml of rHuTNF-a.
Solubility: It is recommended to reconstitute the lyophilized TNFR2 in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com