Recombinant human TFF3 produced in
E. coli is a single, non-glycosylated, polypeptide chain containing 69 amino acids (22-80 a.a.) which includes a 10 amino acid His-Tag fused at N-terminus and having a total molecular mass of 7.82 kDa. TFF3 is purified by proprietary chromatographic techniques.
Synonyms: TFF-3, ITF, TFI, HITF, hP1. B, TFF3, Trefoil factor 3, Intestinal trefoil factor.
Formulation: The TFF3 protein was lyophilized from 0.4μm filtered solution at a concentration of 0.5 mg/ml containing 20 mM Tris pH 7.5 and 150 mM NaCl.
Physical Appearance: White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: MKHHHHHHAS EEYVGLSANQ CAVPAKDRVD CGYpH VTPKE CNNRGCCFDS RIPGVPWCFK PLQEAECTF.
Uniprot ACC number: Q07654
Transportation method: Shipped at room temperature.
Stability: Lyophilized TFF3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TFF3 should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Solubility: Add deionized water to a working concentration approximately 0.5 mg/ml and let the Lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by appropriate sterile filter before using it in the cell culture.
Safety data sheet: Inquire at info@3hbiomedical.com