Recombinant human TFF-3 produced in E. coli is a homodimeric, non-glycosylated, polypeptide chain containing 2 x 59 amino acid chains which includes a 40 amino acid trefoil motif containing 3 conserved interamolecular disulfide bonds and having a total molecular mass of 13.2 kDa. TFF-3 is purified by proprietary chromatographic techniques.
Synonyms: TFF-3, ITF, TFI, HITF, hP1. B, TFF3, Trefoil factor 3, Intestinal trefoil factor.
Formulation: lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 97% as determined by RP-HPLC and SDS-PAGE analysis.
Source:Escherichia coli.
Amino acid sequence: EEYVGLSANQ CAVPAKDRVD CGYPH VTPKE CNNRGCCFDS RIPGVPWCFK PLQEAECTF.
Uniprot ACC number: Q07654
Transportation method: Shipped at room temperature.
Stability: Lyophilized TFF3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TFF3 should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The ED50 as determined by a chemotaxis bioassay using human MCF-7 cells is less than 10 µg/ml, corresponding to a specific activity of > 100IU/mg.
Solubility: It is recommended to reconstitute the lyophilized TFF3 in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com