Recombinant human TFF-1 produced in
E. coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids (25-84) which includes a 10 amino acid His-Tag and having a total molecular mass of 7.9 kDa. TFF-1 is purified by proprietary chromatographic techniques.
Synonyms: TFF-1, TFF1, pS2, BCEI, HPS, HP1. A, pNR-2, D21S21, pS2 protein, Trefoil factor 1, Breast cancer estrogen-inducible protein.
Formulation: The TFF1 His-Tag protein was lyophilized from 0.4μm filtered solution at a concentration of 0.5 mg/ml containing 20 mM Tris pH 7.5 and 20 mM NaCl.
Physical Appearance: Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as determined by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: MKHHHHHHAS EAQTETCTVA PRERQNCGFP GVTPSQCANK GCCFDDTVRG VPWCFYPNTI DVPPEEECEF.
Uniprot ACC number: P04155
Transportation method: Shipped at room temperature.
Stability: Lyophilized TFF1 His-Tag although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TFF1 His-Tag should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Solubility: It is recommended to add deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the Lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Safety data sheet: Inquire at info@3hbiomedical.com