Recombinant human TGFA (40-89) produced in
E. coli is a single, non-glycosylated, polypeptide chain containing 50 amino acids and having a molecular mass of 5.6 kDa. The TGFA is purified by proprietary chromatographic techniques.
Synonyms: Transforming Growth Factor Alpha, Protransforming Growth Factor Alpha, TGF-Alpha, TGFA.
Formulation: lyophilized from a 0.2µm filtered solution in 0.1% TFA.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as determined by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: VVSHFNDCPD SHTQFCFHGT CRFLVQEDKP ACVCHSGYVG ARCEHADLLA.
Uniprot ACC number: P01135
Transportation method: Shipped at room temperature.
Stability: Lyophilized TGFA although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TGFA should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The ED50, as measured in a proliferation assay using mouse BALB/c 3T3 cells, is 0.395 ng/ml.
Solubility: It is recommended to reconstitute the lyophilized TGFA in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com