Recombinant human Soluble TNF-related apoptosis-inducing ligand produced in
E. coli is a single, non-glycosylated polypeptide chain containing 169 amino acids (114-281) and having a molecular mass of 19.6 kDa. The sTRAIL is purified by proprietary chromatographic techniques.
Synonyms: Tumor necrosis factor ligand superfamily member 10, TNF-related apoptosis-inducing ligand, Protein TRAIL, Apo-2 ligand, Apo-2L, CD253 antigen, TL2, APO2L, TNFSF10.
Formulation: TRAIL protein solution (1mg/ml) containing 20 mM Tris-HCl pH 7.5, 300 mM NaCl, 0.1 mM DTT & 10% glycerol.
Physical Appearance: Sterile Filtered colorless liquid.
Purity: Greater than 95% as determined by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: MVRERGPQRV AAHITGTRGR SNTLSSPNSK NEKALGRKIN SWESSRSGHS FLSNLHLRNGELVIHEKGFY YIYSQTYFRF QEEIKENTKN DKQMVQYIYK YTSYPDPILL MKSARNSCWSKDAEYGLYSI YQGGIFELKE NDRIFVSVTN EHLIDMDHEA SFFGAFLVG.
Uniprot ACC number: P50591
Transportation method: Shipped with Ice Packs.
Stability: Store at +4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Biological Activity: Measured in a cell cytotoxicity assay using Jurkat human T Lymphocyte. The ED50 for this effect is less or equal to 1 ng/ml
.
Safety data sheet: Inquire at info@3hbiomedical.com