Recombinant human Stem Cell Factor produced in insect cells is a single, glycosylated polypeptide chain containing 165 amino acids and having a molecular mass of 18.4 kDa. The SCF is fused to a C-terminal His-tag (6xHis) and purified by proprietary chromatographic techniques.
Synonyms: Kit ligand Precursor, C-kit ligand, SCF, Mast cell growth factor, MGF, SF, KL-1, Kitl, DKFZp686F2250.
Formulation: The protein is supplied in 1xPBS, pH 7.4.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 98% as determined by analysis by SDS-PAGE.
Source: Sf9, Insect Cells.
Uniprot ACC number: P21583
Transportation method: Shipped at room temperature.
Stability: Lyophilized KIT ligand although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution SCF should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The ED50 as determined by the dose-dependent stimulation of Human TF-1 cells is typically 1-5 ng/ml
.
Solubility: It is recommended to reconstitute the lyophilized Stem Cell Factor in 10 mM acetic acid not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com