Recombinant human SCF produced in HEK cells is a glycosylated monomer, having a molecular weight range of 35-45 kDa due to glycosylation. The SCF is purified by proprietary chromatographic techniques.
Synonyms: Kit ligand Precursor, C-kit ligand, SCF, Mast cell growth factor, MGF, SF, KL-1, Kitl, DKFZp686F2250.
Formulation: SCF was lyophilized from a 0.2 µm filtered solution (1mg/ml) containing 1xPBS.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as obsereved by SDS-PAGE.
Source: HEK.
Uniprot ACC number: P21583
Transportation method: Shipped at room temperature.
Stability: Lyophilized SCF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution SCF should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The specific activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line). The EC50 is 15.25 ng/ml.
Solubility: It is recommended to reconstitute the lyophilized SCF in sterile PBS containing 0.1% endotoxin-free recombinant HSA.
Safety data sheet: Inquire at info@3hbiomedical.com