Recombinant human Stem Cell Factor produced in
E. coli is a single, non-glycosylated polypeptide chain containing 165 amino acids and having a molecular mass of 18.4 kDa. The SCF is purified by proprietary chromatographic techniques.
Synonyms: Kit ligand Precursor, C-kit ligand, SCF, Mast cell growth factor, MGF, SF, KL-1, Kitl, DKFZp686F2250.
Formulation: lyophilized from a concentrated (1mg/ml) solution in water containing 0.02% NaHCO3.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 98% as determined by: (a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Gly-Ile-Cys.
Uniprot ACC number: P21583
Transportation method: Shipped at room temperature.
Stability: Lyophilized KIT ligand although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution SCF should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The ED50 as determined by the dose-dependant stimulation of Human TF-1 cells is < 2 ng/ml, corresponding to a Specific Activity of 500,000 IU/mg.
Solubility: It is recommended to reconstitute the lyophilized Stem Cell Factor in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
References: 1. Title:Direct binding of Cbl to Y568 and Y936 of the stem cell factor receptor/c-Kit is required for ligand-induced ubiquitination, internalization and degradation. Publication:Biochemical Journal I mMediate Publication. Published on 19 Jun 2006 as manuscript BJ20060464. Link:http://www. biochemj. org/bj/imps_x/pdf/BJ20060464. pdf2. Title:The D816V Mutation of c-Kit Circumvents a Requirement for Src Family Kinases in c-Kit Signal Transduction. Publication: March 5, 2009, doi: 10.107 4/jbc. M808058200 April 24, 2009 The Journal of Biological Chemistry, 284, 11039-11047. Link:http://www. jbc. org/content/284/17/11039. full3. Title:Structural Basis for c-KIT Inhibition by the Suppressor of Cytokine Signaling 6 (SOCS6) Ubiquitin Ligase. Publication:First Published on October 28, 2010, doi: 10.107 4/jbc. M110.173526 January 7, 2011 The Journal of Biological Chemistry, 286, 480-490. Link:http://www. jbc. org/content/286/1/480. full4. Title: Gab2 is involved in differential pH ospH oinositide 3-kinase signaling by two splice forms of c-KitPublications: Journal of Biological Chemistry 283.41 (2008): 27444-27451. Link: http://www. jbc. org/content/283/41/27444. full
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