Recombinant human Sonic HedgeHog Recombinant Human produced in
E. coli is a single, non-glycosylated polypeptide chain containing 179 amino acids and having a molecular mass of 20.1 kDa. The Cys at position 2 has been substituted with 2 Ile’s.
Synonyms: SHH, HHG-1, HHG1, Sonic hedgehog protein, TPT, HLP3, HPE3, S mMCI, TPTPS, MCOPCB5.
Formulation: SHH is lyophilized from 10 mM Na3PO4, pH 7.5.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 97% as determined by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: MIIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRALDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGGCFP
Uniprot ACC number: Q15465
Transportation method: Shipped at room temperature.
Stability: Lyophilized Human Sonic HedgeHog although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Sonic HedgeHog should be stored at +4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Biological Activity: The activity is measured by the dose-dependent induction of alkaline pH ospH atase production by C3H/10T1/2 (CCL-226) fibroblasts and is 1.2-1.8ug/ml corresponding to a specific activity of 800U/mg.
Solubility: It is recommended to reconstitute the lyophilized SHH in sterile 10 mM Na3PO4 pH 7.5 not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com