Recombinant human SAA1 produced in
E. coli is a single, non-glycosylated polypeptide chain containing 104 amino acids and having a molecular mass of 11.7 kDa. The SAA1 is purified by proprietary chromatographic techniques.
Synonyms: Serum amyloid A protein, SAA, Amyloid protein A, Amyloid fibril protein AA, SAA1, SAA2, PIG4, TP53I4, MGC111216.
Formulation: SAA1 was lyophilized from a 0.2µm filtered concentrated solution in 20 mM Tris-HCl, pH 9.0 and 150 mM NaCl.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 97% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: RSFFSFLGEA FDGARDMWRA YSDMREANYI GSDKYFHARG NYDAAKRGPG GVWAAEAISD ARENIQRFFG HGAEDSLADQ AANEWGRSGK DPNHFRPAGL PEKY.
Uniprot ACC number: P0DJI8
Transportation method: Shipped at room temperature.
Stability: Lyophilized SAA1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution SAA1 should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The biological activity determined by a chemoattract bioassay using human monocytes is in a concentration range of 10-100 ng/ml.
Solubility: It is recommended to reconstitute the lyophilized SAA1 in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com