Recombinant human RANK produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 427 amino acids (28-212 a.a.) and having a molecular mass of 47.6 kDa. (Molecular size on SDS-PAGE will appear at approximately 40-57 kDa). RANK is expressed with a 242 amino acid hIgG-His-tag at C-Terminus and purified by proprietary chromatographic techniques.
Synonyms: Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator, Loss Of Heterozygosity, 18, Chromosomal Region 1, Osteoclast Differentiation Factor Receptor, Receptor Activator Of NF-KB, Paget Disease Of Bone 2, ODFR, RANK, Tumor Necrosis Factor Receptor Superfamily, Member 11a, Activator Of NFKB, Receptor Activator Of Nuclear Factor-Kappa B, CD265 Antigen, LOH18CR1, TRANCER, CD265, OPTB7, OSTS, PDB2, FEO, OFE, Tumor necrosis factor receptor superfamily member 11A.
Formulation: RANK protein solution (1mg/ml) contains phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Physical Appearance: Sterile Filtered colorless solution.
Purity: Greater than 90% as determined by SDS-PAGE.
Source: Sf9, Baculovirus cells.
Amino acid sequence: ADPLQIAPPC TSEKHYEHLG RCCNKCEPGK YMSSKCTTTS DSVCLPCGPD EYLDSWNEED KCLLHKVCDT GKALVAVVAG NSTTPRRCAC TAGYHWSQDC ECCRRNTECA PGLGAQHPLQ LNKDTVCKPC LAGYFSDAFS STDKCRPWTN CTFLGKRVEH HGTEKSDAVC SSSLPARKPP NEpH VYLPLE PKSCDKTHTC PPCPAPELLG GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRD ELTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPGKHHHHHH.
Uniprot ACC number: Q9Y6Q6
Transportation method: Shipped with Ice Packs.
Stability: Store at +4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Safety data sheet: Inquire at info@3hbiomedical.com