Recombinant human Prolactin Antagonist des 1-9, G129R produced in
E. coli is a single, non-glycosylated polypeptide chain containing 190 amino acids + an additional Ala at n-terminal and having a molecular mass of ~ 22 kDa. The Human Prolactin Antagonist is purified by proprietary chromatographic techniques.
Synonyms: Ma mMotropin, Luteotropic hormone, Luteotropin, PRL.
Formulation: The protein was lyophilized from a concentrated (1mg/ml) solution with 0.02% NaHCO3
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as determined by gel filtration analysis.
Source:Escherichia coli. Amino acid sequence: The a.a.. sequence of the 1st 6 N-terminal a.a.. was found to be Ala-Arg-Ser-Gln-Val-Thr.
Uniprot ACC number: P01236
Transportation method: Shipped at room temperature.
Stability: Lyophilized Prolactin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Prolactin should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: Human Prolactin Antagonist was tested for its biological functionality in-vitro by inducing proliferation of Nb2 cells or Baf/3 cells that were stably transfected with Human Prolactin receptors. Prolactin Antagonist also interacts at 1:1 molar ratio with human prolactin receptor extracellular domain as documented by SEC and SPR (Biacore analysis).
Solubility: It is recommended to reconstitute the lyophilized Prolactin in sterile 0.4% NaHCO3 pH 8.5, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com